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. 2017 Jun 28;8:1188. doi: 10.3389/fmicb.2017.01188

Table 1.

Primers and reaction profile for Trichomonas vaginalis (Pillay et al., 2007).

Primer Target gene Sequence (5′ → 3′) Product (bp)
TVK-3 T. vaginalis-specific repeat DNA fragment ATTGTCGAACATTGGTCTTACCCTC 261
TVK-7 TCTGTGCCGTCTTCAAGTATGC

Reaction profile: 1 cycle → 94°C for 1 min; 35 cycles → 94°C for 1 min, 60°C for 30 s, and 72°C for 1 min; 1 cycle → 72°C for 5 min; 1 cycle → 0°C for ∞.