Table 1.
Primer | Target gene | Sequence (5′ → 3′) | Product (bp) |
---|---|---|---|
TVK-3 | T. vaginalis-specific repeat DNA fragment | ATTGTCGAACATTGGTCTTACCCTC | 261 |
TVK-7 | TCTGTGCCGTCTTCAAGTATGC |
Reaction profile: 1 cycle → 94°C for 1 min; 35 cycles → 94°C for 1 min, 60°C for 30 s, and 72°C for 1 min; 1 cycle → 72°C for 5 min; 1 cycle → 0°C for ∞.