Table 3.
Primers and reaction profile for quantitative PCR of M. hominis and M. genitalium, U. urealyticum and U. parvum (Olsen et al., 2009; Ferandon et al., 2011; Frolund et al., 2014).
| Primer | Target gene | Sequence (5′ → 3′) | Species |
|---|---|---|---|
| MHyidCfwd | yidC | TCACTAAACCGGGTATTTTCTAACAA | M. hominis |
| MHyidCrev | TTGGCATATATTGCGATAGTGCTT | ||
| Probe MHyidC | FAM-CTACCAATAATTTTAATATCTGTCGGTATG-MGB | ||
| MgPa-355F | MgPa | GAGAAATACCTTGATGGTCAGCAA | M. genitalium |
| MgPa-432R | GTTAATATCATATAAAGCTCTACCGTTGTTATC | ||
| Probe MgPa-380 | Urease gene | FAM-ACTTTGCAATCAGAAGGT-MGB | U. urealyticum |
| U195F | GCAAGAAGACGTTTAGCTAGAGGTTT | ||
| U8R | CACGAGCAGATTGCATTAAGTCAG | ||
| Probe U8 | FAM-TAATTACTGACCACGTAGTGGA-MGB | ||
| U195F | Urease gene | GCAAGAAGACGTTTAGCTAGAGGTTT | U. parvum |
| U3R | CGAGCAGATTGCATTAGGTCAG | ||
| Probe U3 | FAM-TTTAATTACTGATCATGTAATGGA-MGB |
Reaction profile: 1 cycle → 50°C for 1 min 94°C for 10 min; 45 cycles → 95°C for 15 s, 60°C for 1 min and 72°C for 1 min.