Table 3.
List of Nonnuclear and Unknown Group Proteins Used for GFP Fusion Protein Localization Analysis
| Group | Clone Name | DDBJ Accession Number | Clone Statusa | Hit Protein by Homology Search (Protein ID) | Predicted NLS by PSORT Searchb | NLS Score | MW of Fusion Proteinc | Nuclear Transport Efficiency | Corresponding Photograph |
|---|---|---|---|---|---|---|---|---|---|
| Nonnuclear proteins | 12FPB009 | AB110164 | Full | O. sativa putative fructokinase II protein (AAL26573.1) | n.f. | 0.0 | 62,934 | + | Figure 2J |
| 12FPR057 | AB110168 | Full | O. sativa protein similar to Zea mays caffeoyl-CoA 3-O-methyltransferase (BAA81774.1) | n.f. | 0.0 | 57,199 | ++ | Figure 2D | |
| 12YPR001 | AB110170 | Full | O. sativa putative thiamine biosynthesis protein (BAC45141.1) | n.f. | 0.0 | 66,344 | − | n.s.d | |
| 13YPB030 | AB110173 | Full | O. sativa putative ankyrin protein (AAG46181.1) | n.f. | 0.0 | 58,360 | ++ | Figure 2B | |
| 13YPG038 | AB110177 | Full | O. sativa katanin p60 subunit A 1-like protein (BAB90151.1) | Position 78-85 (RGKGFVDS) | 0.1 | 74,015 | − | n.s. | |
| 13YPG044 | AB110178 | Full | O. sativa putative nascent polypeptide associated complex α chain protein (BAB90246.1) | n.f. | 0.0 | 50,742 | − | n.s. | |
| 13YPR038 | AB110180 | Full | O. sativa Rubisco activase large isoform precursor protein (BAA97583.1) | n.f. | 0.0 | 76,859 | − | n.s. | |
| 14FPG037 | AB110186 | Full | O. sativa putative dihydroflavonol reductase protein (AAD24584.1) | n.f. | 0.0 | 65,110 | − | n.s. | |
| 21C4AB003 | AB110191 | Full | O. sativa class I low-molecular-weight heat shock protein 17.9 protein (AAK54445.1) | n.f. | 0.0 | 48,703 | + | Figure 2F | |
| 22C4AG009 | AB110193 | Full | O. sativa Asp aminotransferase protein (BAA03504.1) | n.f. | 0.0 | 73,960 | + | Figure 2H | |
| Unknown proteins | 12FPG037 | AB110165 | Full | O. sativa protein (BAA93034.1) | n.f. | 0.0 | 56,082 | +/− | n.s. |
| 12FPG084 | AB110166 | Partial | Arabidopsis F28N24.14 protein (AAF88121.1) | n.f. | 0.0 | 56,599 | +/− | n.s. | |
| 12FPR024 | AB110167 | Full | Arabidopsis dehydrogenase-like protein (BAB01322.1) | Position 111-118 (KGGAFVEA) | 0.1 | 61,787 | + | Figure 3H | |
| 12YPG019 | AB110169 | Full | Arabidopsis AT3g53990 protein (AAG40033.1) | n.f. | 0.0 | 49,223 | + | n.s. | |
| 13YPB021 | AB110171 | Full | O. sativa unknown protein (AAK14411.1) | n.f. | 0.0 | 44,266 | ++ | n.s. | |
| 13YPB022 | AB110172 | Full | Arabidopsis ERD15 protein (BAA06384.1) | n.f. | 0.0 | 49,239 | ++ | Figure 3F | |
| 13YPB053 | AB110174 | Partial | Arabidopsis putative protein F9K21.210 (CAB75492.1) | n.f. | 0.0 | 57,224 | ++ | n. s. | |
| 13YPG010 | AB110175 | Full | Arabidopsis putative protein T25B15-70 (CAC07921.1) | n.f. | 0.0 | 49,538 | + | Figure 3K | |
| 13YPG014 | AB110176 | Full | Arabidopsis putative protein F17A8.180 (CAB39650.1) | Position 3-6 (RKRK) | 0.3 | 54,315 | ++ | Figure 3C | |
| 13YPR033 | AB110179 | Full | Arabidopsis F2J10.8 protein (AAF76441.1) | n.f. | 0.0 | 78,095 | + | n.s. | |
| 13YPR054 | AB110181 | Partial | Arabidopsis putative dehydrogenase protein (AAF14837.1) | n.f. | 0.0 | 57,430 | − | n.s. | |
| 14FPB004 | AB110182 | Full | O. sativa YY2 protein (BAA23618.1) | n.f. | 0.0 | 72,412 | +/− | n.s. | |
| 14FPB010 | AB110183 | Full | Arabidopsis putative protein AT4g25840 (CAB39605.1) | n. f. | 0.0 | 56,046 | ++ | Figure 3D | |
| 14FPB077 | AB110184 | Full | Arabidopsis putative protein T30N20 130 (CAB96841.1) | n.f. | 0.0 | 51,163 | ++ | Figure 3E | |
| 14FPB137 | AB110185 | Full | Arabidopsis unknown protein F11B9.13 (AAG50959.1) | n.f. | 0.0 | 57,062 | + | n.s. | |
| 15FPR003 | AB110187 | Partial | Arabidopsis F8M12.21 protein (AAC33943.1) | n.f. | 0.0 | 62,124 | + | n.s. | |
| 16FPG004 | AB110188 | Full | Arabidopsis protein F14013.20 (BAB03019.1) | Position 248-251 (KKRK) | 0.3 | 57,715 | ++ | Figure 3A | |
| 17FPG011 | AB110189 | Full | Arabidopsis aluminum-induced protein-like protein (BAB11312.1) | n.f. | 0.0 | 56,779 | + | Figure 3J | |
| 17YPR003 | AB110190 | Full | Arabidopsis protein MCK7.9 (BAA96913.1) | n.f. | 0.0 | 66,059 | + | n.s. | |
| 21C4BG001 | AB110192 | Full | Arabidopsis protein MIJ24.6 (BAB08888.1) | Position 352-355 (RKKH) | 0.7 | 71,032 | + | Figure 3L | |
| 22C4AG010 | AB110194 | Partial | O. sativa B1153F04.12 protein (BAB62605.1) | n.f. | 0.0 | 45,826 | + | Figure 3I | |
| 23C4AG017 | AB110195 | Full | O. sativa P0038C05.1 protein (BAB19328.1) | n.f. | 0.0 | 39,018 | ++ | Figure 3G | |
| 23C4AG047 | AB110196 | Full | Arabidopsis protein K5F14.2 (BAB09934.1) | Position 84-87 (KRPR) | 0.3 | 56,461 | ++ | Figure 3B | |
| 23C4AG056 | AB110197 | Partial | Arabidopsis unknown protein AT4g37210 (AAK44004.1) | n.f. | 0.0 | 65,177 | ++ | n.s. | |
| 23C4AR004 | AB110198 | Full | Arabidopsis protein MTE24.1 (BAB01311.1) | n.f. | 0.0 | 46,905 | + | n.s. | |
| 23C4BG006 | AB110199 | Partial | Arabidopsis putative protein F14L2-150 (CAB88542.1) | n.f. | 0.0 | 48,195 | ++ | n.s. |
a
“Full” and “partial” indicate probable full-length and partial open reading frame encoding clones, respectively.
b
Numbers are calculated NLS position in each full-length protein. n.f., not found by PSORT search.
c
MW, molecular weight.
d
n.s., data not shown.