Complementation of the hog1-1 Mutation.
(A) RNA gel blots of total RNA prepared from the L5 (lane 1), L5-hog1-1 (lane 2), and L5-hog1-1-complementation (lane 3) lines. The probes are indicated at the bottom of the panel.
(B) DNA gel blots of total genomic DNA prepared from L5 (lane 1), L5-hog1-1 (lane 2), and L5-hog1-1-complementation lines (lane 3) cut with methylation-sensitive restriction endonucleases as indicated. The DNA was also cut with HindIII. The probes are indicated at the bottom of the panel, and the numbers at left are the molecular weights in kilobases.