. 2005 Feb;17(2):404–417. doi: 10.1105/tpc.104.028332

Table 1.

Interactions among CHS Silencing, the V- and C-Inserts, and Nine Putative Genetic Modifiers of Gene Silencing

		C-Insert				V-Insert
Gene	Allele	Green	Purple	χ_3:1²	χ_9:7²	Green	Purple	χ_3:1²	χ_9:7²
SGS1	sgs1-1	74	23	0.09^NS	15.83^S	123	33	1.23^NS	32.37^S
SGS2	sgs2-1	73	27	0.21^NS	11.40^S	115	49	2.08^NS	12.82^S
SGS3	sgs3-1	73	24	0.00^NS	14.24^S	126	35	0.91^NS	31.70^S
AGO1	ago1-27	68	20	0.24^NS	15.80^S	117	39	0.00^NS	22.29^S
KYP	kyp-2	32	16	1.78^NS	2.12^NS	66	16	1.32^NS	19.57^S
DDM1	ddm1-2	42	41	26.35^S	1.08^NS	93	28	0.22^NS	20.88^S
MET1	met1-1	43	37	19.27^S	0.20^NS	82	25	0.15^NS	18.07^S
CMT3	cmt3-7	47	36	14.94^S	0.00^NS	69	20	0.30^NS	16.37^S
HOG1	hog1-1	46	34	13.07^S	0.05^NS	68	18	0.76^NS	18.20^S
WT	–	64	27	1.06^NS	7.33^S	64	19	0.20^NS	14.67^S

The results presented are the segregations of green (CHS silenced) and purple (CHS active) seedlings in the F2 generation derived from crosses homozygous for the respective insert and mutant. The inserts are dominant and the modifiers are (when effective) recessive in the F2 generation. Failure of a putative modifier mutation to affect CHS silencing results in a 3:1 (green:purple) ratio because of the segregation of the insert alone. Success of a putative modifier mutation in relieving C-insert silencing results in a 9:7 (green:purple) ratio because of the superimposition of the two independent segregations. Each set of observations was tested for fit to each ratio using corrected χ². Values of χ² > 3.84 indicate <5% frequency by chance alone and are marked as significant (^S), and values lower than this are marked as not significant (^NS).