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Determination of Methylation Levels in Total Genomic DNA in the Wild Type and a Variety of Homozygous Mutantsa
| CpG Methylation in TaqI Sites of Genomic DNA
| ||||
|---|---|---|---|---|
| Wild Type | hog1-1 | hog1-2 | hog1-3 | ddm1-2 |
| 5-Methyl-cytosine percentage of labeled cytosine | ||||
| 23.0 ± 2.8 | 10.3 ± 0.6 | 16.7 ± 1.0 | 15.8 ± 2.2 | 7.5 ± 1.1 |
| 5-Methyl-cytosine percentage of wild-type level | ||||
| 100.0 | 44.7 | 72.6 | 68.7 | 32.6 |
The method employed measures DNA methylation in the context CpG of the TaqI site. Values are averages ± se (n = 5).
