Figure 1.

A) Molecular structures of APPA, cisplatin and picoplatin. B) Anti‐proliferative activity of cisplatin derivatives against U2OS cells. C) Schematic representation of a DNA hairpin (hp), a 1,2‐GG intra‐strand DNA crosslink (hp‐Pt) and chemical labeling of DNA‐Pt (hp‐Pt‐488). For clarity, a single regioisomer is shown for hp‐Pt‐488. D) Mass spectrometry detection of a free DNA hairpin, the corresponding DNA‐Pt and its labeled counterpart. hp‐Pt was observed as the molecular ion peak with loss of nitrogen. E) Molecular structure of APP and detection of genomic DNA platination by dot blot. DNA samples were purified from U2OS cells treated with APP (250 μm) or APPA (250 μm) for 3 h. EtBr (Ethidium bromide) stained gel of input DNA used as loading control.