Figure 6.

KNOX Immunolocalization in S. saxorum.

(A) A vegetative apex of S. saxorum showing leaves in whorls of three. New leaves are initiated directly opposite a leaf in the preceding whorl (bar = 1 mm).

(B) Transverse section of apex. Nuclear-localized KNOX proteins can be seen in the central, triangular SAM and in leaf veins (bar = 50 μm).

(C) and (D) Longitudinal sections of different S. saxorum apices, showing staining in the central SAMs, SAMs in the axils of leaves (star), and in developing veins. An unstained region was detected at the site of leaf initiation from the SAM (arrow in [D]). Bar = 100 μm.

(E) to (J) Transverse sections through leaves at increasing stages of maturity. Arrows indicate vascular localization of KNOX proteins. Bars = 25 μm in (E), 50 μm in (F) and (G), and 100 μm in (H) to (J).