Table 1.

Binding affinities and helicity for peptides determined by competitive fluorescence polarisation and circular dichroism, respectively. Errors shown are the standard deviation of two triplicate experiments.

Peptide[a]	FP K d [nm]	Helicity [%]	Peptide[a]	FP K d [nm]	Helicity [%]
SP0	8.6±1.7	52
SP1 (A)	4.5±1.3	53	SP1‐K (A)	5.5±1.2	55
SP1 (B)	12.0±1.6	31	SP1‐K (B)	12.0±1.7	31
SP2 (A)	3.8±1.0	67	SP2‐K (A)	11.1±2.4	65
SP2 (B)	30.2±6.6	35	SP2‐K (B)	14.7±2.0	36
			nutlin‐3 a	110±26.8	n.a.

[a] CD and direct FP data for SP1‐βA‐F (A/B) and SP2‐βA‐F (B) are provided in the Supporting Information. n.a.: not applicable.