Figure 2.

Purification of Recombinant VLN1.

(A) Coomassie Blue-stained protein gel of recombinant VLN1 purification. Lane 1, Total extract from bacterial cells (20 μg); lane 2, (NH₄)₂SO₄ precipitate (15 μg); lane 3, DEAE-Sephacel eluate (8 μg); lane 4, Cibacron Blue 3G-A eluate (4 μg); lane 5, Q-Sepharose eluate (2 μg). The migration of molecular mass (MM) standards is given at the left in kilodaltons.

(B) Protein immunoblot of purified VLN1 (100 ng) probed with affinity-purified, anti-VLN1(G1–G3) antibody.