Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Feb;17(2):486–501. doi: 10.1105/tpc.104.028555

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, American Society of Plant Biologists

PMC Copyright notice

Figure 7. — VLN1 Stabilizes Actin Filaments against ADF.

Conditions: 10 mM Tris-HCl, pH 8, 50 mM KCl, 2 mM MgCl₂, 1 mM EGTA, 0.2 mM ATP; 0.2 mM CaCl₂, 0.5 mM DTT, and 3 mM NaN₃ at 20°C.

(A) Time course of actin filament depolymerization monitored by pyrene fluorescence. Open squares, 1 μM actin filaments alone; open diamonds, 1 μM F-actin with 8 μM latrunculin B (LatB); open circles, 1 μM F-actin with 8 μM LatB and 1 μM VLN1; crosses, 1 μM F-actin with 0.6 μM ADF1, 8 μM LatB and 1 μM VLN1; and open triangles, 1 μM F-actin with 0.6 μM ADF1 and 8 μM LatB.

(B) Time course of actin polymerization (4 μM) monitored by pyrene fluorescence. Dashed line with no symbols, no additions to actin; open circles, actin with 1 μM VLN1; closed squares, actin with 1 μM ADF1; and open squares, actin with 1 μM VLN1 and 1 μM ADF1.