Table 4.
The Names and Sequences of the Oligonucleotide Primers That Were Used in This Article
| Name | Sequence (5′−3′) |
|---|---|
| Primers used for DIS3 allele sequencing: | |
| DIS3-F1 | CTCATATGCCAACACAAGATCCTCCA |
| DIS3-F2 | TCCGCCGTCTTGAATCCAC |
| DIS3-F3 | GTGATGAAGGTTTGCTGCTGAG |
| DIS3-F4 | GTTTGAAACGCTACACCGTACC |
| DIS3-F5 | GGAGGAGCATTTAGAGGCTCAT |
| DIS3-F6 | GACAATTATGTGGACGCACCAG |
| DIS3-F7 | GCATCAGAAGTCGTTGAAACTTCT |
| DIS3-F8 | CTCTGGATGATGAAACCGATTGT |
| DIS3-F9 | CTGATGCTGGAGAGATTTTCCAT |
| DIS3-F10 | CCTGAGACTATTCCGCCCAAT |
| DIS3-F11 | GTCGATGCTGTTGCAGCTCATGA |
| DIS3-F12 | CCCAGATGGTCCTGTGAACAGA |
| DIS3-F13 | AATCGATGATCGTTGGCTTTTC |
| DIS3-R1 | GGTGGTGCCAGCCGAGAA |
| DIS3-R2 | CCAATGACTGTATGTATTTAATGTTATATGT |
| DIS3-R3 | CGTGTTGTTACTGCCGGCTTTAG |
| DIS3-R4 | GATCATCGCTTGAACTTTGAGATT |
| DIS3-R5 | CCTGCTGATTGTGGTTCAAACTCTAA |
| DIS3-R6 | CAACTGTATCATGGAAAATCTCTCC |
| DIS3-R7 | CACAATCGGTTTCATCATCCAG |
| DIS3-R8 | GGAGAAGTTTCAACGACTTCTGAT |
| DIS3-R9 | CTGTTCCTGTTTCCGACTCCATG |
| DIS3-R10 | TGATGAGCCTCTAAATGCCCTC |
| DIS3-R11 | AGCAATCTAACCTTTGTTAGAGAA |
| DIS3-R12 | CATCTCAGCAGCAAACCTTCAT |
| DIS3-R13 | CAAGACGGCGGATATAGGTAGG |
| Primers used to make different plasmid constructs: | |
| pTOPO-DIS3-WA/F | CACCATGGAGCCTAAGCAAGAGTTGAATCTG |
| pTOPO-DIS3-WA/R | GAATTCTCAAGAATCATCCAACTATCTG |
| pTOPO-DIS3-ΔWA/F | CACCATGGAGCCTAAGCAAGAGTTGAATCTG |
| pTOPO-DIS3-ΔWA/R | GAATTCTCACTTTGTTGATTGAGTTGC |
| pTOPO-HSWAVE1/F | CACCATGGACATGCCGCTAGTGAAAAGAAACATCG |
| pTOPO-HSWAVE1/R | GGATCCTTACTCCAACCAATCTACTTCATC |
| pTOPO-DIS3-SHD/F | CACCAGAATTCACATGCCGTTGACGAGGTACCAATC |
| pTOPO-DIS3-SHD/R | CTCGAGCCATTCCTCCACTGAGATGCTCTCC |
| pEN-ATARPC3/F | CACCATGGTTTATCACTCGAGTTTTGT |
| pEN-ATARPC3/R | GAATTCTCATAGGACGACCACGTTCA |
| pEN-ATABIL1/F | CACCGAATTCATGGAAACGGAGATAT |
| pEN-ATABIL1/R | GGATCCTCACGAGACATAGCCAGCTT |
| pEN-1084WA/F | CACCGGATCCGTCGAAACCAGTAGTTCTGCTTTATCAGC |
| pEN-1084WA/R | CCATCTCGAGTCAAGAATCACTCCAACTATCTGAATCTTC |
| Primers used for gene expression study (RT-PCR): | |
| ATSCAR1-Nterm/F | CACCAGAATTCACATGTATGACGAATTGCCACCG |
| ATSCAR1-Nterm/R | CTCGAGGTTTAGCCACAGAGGCAAGTCTTG |
| DIS3-Nterm/F (An) | CACCAGAATTCACATGCCGTTGACGAGGTACCAATC |
| DIS3-Nterm/R | CTCGAGCCATTCCTCCACTGAGATGCTCTCC |
| ATSCAR3-Nterm/F | CACCATGGAATGCCACGGAATGTATACGG |
| ATSCAR3-Nterm/R | GAATTCGGCCAAACGTGACATATCTCAAC |
| ATSCAR4-Nterm/F | CACCGAATTCATGGCATTGACGAGATACCAG |
| ATSCAR4-Nterm/R | CTCGAGCGTCTCTTTAATAGTTGTGTGAG |
| ATSCAR5-Nterm/F | CACCATGGACATGCCGTTGGTGAGGTTCAAG |
| ATSCAR5-Nterm/R | GGATCCCACTTTGTTCTTGCGCACCTGAC |
| ATPIR (SRA-1)/F | CGCCTTCTGACTCTTCCAGC |
| ATPIR (SRA-1)/R | CCATTGTCCAAGGCAAAGAGAA |
| pEN-ATABIL1/F | CACCGAATTCATGGAAACGGAGATAT |
| pEN-ATABIL1/R | GGATCCTCACGAGACATAGCCAGCTT |
| ATARPC2/F | ATGATACTATTGCAGTCACATTCA |
| ATARPC2/R | AACTTGACGTGATAACTAACATAAG |
| ATARPC4/F | TAGGACATGGCAAACTCATTAGGG |
| ATARPC4/R | ACTGGCTTCCTCCTCAGTACTTGAAA |
| GAPC/F | CACTTGAAGGGTGGTGCCAAG |
| GAPC/R | CCTGTTGTCGCCAACGAAGTC |