Figure 3.

Expression of Mutant HOT1-4 Protein in Wild-Type Plants Recapitulates the 38°C Sensitivity Phenotype of hot1-4.

The hot1-4 genomic DNA under its own promoter was expressed in transgenic wild-type plants. Two independent transgenic lines (T3:1 and T3:2) are shown compared with the wild type and hot1-4 (three seedlings each). The hypocotyl elongation assay was performed as in Figure 1C. Equal protein samples from heat-stressed seedlings (38°C for 90 min followed by 2 h at 22°C) were analyzed with the indicated antisera against AtHsp101, sHsps, or glyceraldehyde-3-phosphate dehydrogenase (GAPC).