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Reversal of sHsp Insolubility in the Suppressor Mutants.
Ten-day-old seedlings were treated either at 38°C for 3 h (38) or at 38°C for 90 min followed by 2 h at 22°C and then 60 min at 45°C (45), and total protein (T) was isolated after further recovery for 3 h at 22°C. The insoluble protein fraction (P) was separated from the soluble fraction (S) by centrifugation and analyzed with AtHsp101, AtHsp17.6C-I (sHsp I), and AtHsp17.6C-II (sHsp II) antiserum.
