Table 1.
Genetic Analysis of hot1 Mutants
| Cross | Generation | Total | WT | Semi- dominant | hot | χ2 | P |
|---|---|---|---|---|---|---|---|
| hot1-1 | F1 | 23 | 23 | 0 | 0 | ||
| × WT | F2a | 159 | 127 | 0 | 32 | 1.58 | >0.1 |
| hot1-3 | F1 | 31 | 31 | 0 | 0 | ||
| × WT | F2 | 252 | 187 | 0 | 65 | 0.08 | >0.5 |
| hot1-4 | F1 | 30 | 0 | 30 | 0 | ||
| × WT | F2 | 268 | 64 | 134 | 70 | 0.26 | >0.5 |
| hot1-5 | F1 | 7 | 7 | 0 | 0 | ||
| × WT | F2 | 42 | 33 | 0 | 9 | 0.28 | >0.5 |
| hot1-6 | F1 | 8 | 8 | 0 | 0 | ||
| × WT | F2 | 94 | 72 | 0 | 22 | 0.12 | >0.5 |
| hot1-7 | F1 | 4 | 4 | 0 | 0 | ||
| × WT | F2 | 48 | 38 | 0 | 10 | 0.44 | >0.5 |
The phenotypic scoring was based on the hypocotyl elongation phenotypes of the parental lines after 45°C for 2 h with pretreatment (38°C for 90 min). WT, wild type.
a
The hypocotyl length ranges of the phenotypic classes of F2 progenies were 6.5 to 8 mm for the wild-type phenotype, 2.0 to 4.0 mm for the semidominant phenotype, and 0 mm for hot1-4 mutant phenotype after heat stress.