Table 2.
Genetic Segregation Analysis of Intragenic Suppressors
| No. of plants
|
|||||||
|---|---|---|---|---|---|---|---|
| Genotypes | Total | WT | hot | Sup. | χ2 | P | |
| R223K | F1 | 5 | 5 | 0 | 0 | ||
| × WT | F2 | 36 | 36 | 0 | 0 | ||
| R223K | F1 | 5 | 0 | 0 | 5 | ||
| × hot1-4 | F2 | 36 | 0 | 10 | 26 | 0.14 | >0.9 |
| A270/A305 | F1 | 3 | 3 | 0 | 0 | ||
| × WT | F2 | 38 | 38 | 0 | 0 | ||
| A270/A305 | F1 | 7 | 0 | 0 | 7 | ||
| × hot1-4 | F2 | 40 | 0 | 9 | 31 | 0.13 | >0.9 |
| A297T | F1 | 7 | 7 | 0 | 0 | ||
| × WT | F2 | 36 | 36 | 0 | 0 | ||
| A297T | F1 | 6 | 0 | 0 | 6 | ||
| × hot1-4 | F2 | 29 | 0 | 6 | 23 | 0.73 | >0.5 |
| G313D | F1 | 5 | 5 | 0 | 0 | ||
| × WT | F2 | 36 | 36 | 0 | 0 | ||
| G313D | F1 | 6 | 0 | 0 | 6 | ||
| × hot1-4 | F2 | 32 | 0 | 7 | 25 | 0.16 | >0.9 |
| E319K | F1 | 7 | 7 | 0 | 0 | ||
| × WT | F2 | 32 | 32 | 0 | 0 | ||
| E319K | F1 | 2 | 0 | 0 | 2 | ||
| × hot1-4 | F2 | 28 | 0 | 5 | 23 | 0.76 | >0.5 |
| A329V | F1 | 5 | 5 | 0 | 0 | ||
| × WT | F2 | 46 | 46 | 0 | 0 | ||
| A329V | F1 | 5 | 0 | 0 | 5 | ||
| × hot1-4 | F2 | 39 | 0 | 8 | 31 | 0.14 | >0.9 |
| G384S | F1 | 6 | 6 | 0 | 0 | ||
| × WT | F2 | 36 | 36 | 0 | 0 | ||
| G384S | F1 | 5 | 0 | 0 | 5 | ||
| × hot1-4 | F2 | 32 | 0 | 7 | 25 | 0.16 | >0.9 |
| P388S | F1 | 10 | 10 | 0 | 0 | ||
| × WT | F2 | 40 | 40 | 0 | 0 | ||
| P388S | F1 | 3 | 0 | 0 | 3 | ||
| × hot1-4 | F2 | 40 | 0 | 9 | 31 | 0.13 | >0.9 |
| G611D | F1 | 4 | 4 | 0 | 0 | ||
| × WT | F2 | 46 | 46 | 0 | 0 | ||
| G611D | F1 | 5 | 0 | 0 | 5 | ||
| × hot1-4 | F2 | 43 | 0 | 10 | 33 | 0.07 | >0.9 |
| G649E | F1 | 5 | 5 | 0 | 0 | ||
| × WT | F2 | 45 | 45 | 0 | 0 | ||
| G649E | F1 | 6 | 0 | 0 | 6 | ||
| × hot1-4 | F2 | 44 | 0 | 8 | 36 | 0.36 | >0.5 |
| G653E | F1 | 5 | 5 | 0 | 0 | ||
| × WT | F2 | 37 | 37 | 0 | 0 | ||
| G653E | F1 | 5 | 0 | 0 | 5 | ||
| × hot1-4 | F2 | 34 | 0 | 7 | 27 | 0.35 | >0.5 |
| A723V | F1 | 5 | 5 | 0 | 0 | ||
| × WT | F2 | 27 | 27 | 0 | 0 | ||
| A723V | F1 | 8 | 0 | 0 | 8 | ||
| × hot1-4 | F2 | 35 | 0 | 8 | 27 | 0.08 | >0.9 |
| V813M | F1 | 5 | 5 | 0 | 0 | ||
| × WT | F2 | 46 | 46 | 0 | 0 | ||
| V813M | F1 | 5 | 0 | 0 | 5 | ||
| × hot1-4 | F2 | 48 | 0 | 9 | 39 | 1 | >0.5 |
Phenotype scoring was done by comparing the hypocotyl length distribution patterns of the F2 seedlings with those of the parental lines. The hypocotyl length ranges of the phenotypic classes of F2 progenies were 9.5 to 10.5 mm for the wild-type phenotype, 9.0 to 11.0 mm for the suppressor phenotype (Sup.), and 2.0 to 4.0 mm for hot1-4 mutant phenotype after 38°C heat treatment for 2 h. WT, wild type.