Table 3.
Hypocotyl Elongation Phenotype of Intragenic Suppressors
| Hypocotyl Length (%)a
|
|||
|---|---|---|---|
| Genotype | No. of Isolates | 38°C | 38°C/45°C (%) |
| WT | 91.8 ± 9.6 | 68.0 ± 9.2 (100) | |
| hot1-4 (A499T) | 26.2 ± 4.5 | 0 ± 0 | |
| hot1-3 (null) | 90.8 ± 9.6 | 0 ± 0 | |
| Class 1 suppressors | |||
| A270T/A305T | 6 | 89.9 ± 6.4 | 0 ± 0 |
| E319K | 1 | 84.7 ± 6.6 | 0 ± 0 |
| G384S | 3 | 89.7 ± 8.3 | 0 ± 0 |
| P388S | 3 | 87.5 ± 6.8 | 0 ± 0 |
| G611D | 5 | 90.1 ± 6.1 | 0 ± 0 |
| A723V | 3 | 79.4 ± 9.8 | 0 ± 0 |
| V813M | 2 | 91.4 ± 8.3 | 0 ± 0 |
| Class 2 suppressors | |||
| R223K | 2 | 83.6 ± 5.1 | 28.7 ± 5.8 (42) |
| A297T | 2 | 82.1 ± 9.8 | 29.3 ± 6.3 (43) |
| G313D | 3 | 75.3 ± 6.7 | 9.7 ± 4.1 (14) |
| A329V | 1 | 94.1 ± 6.2 | 51.3 ± 6.7 (75) |
| G649E | 1 | 86.7 ± 8.0 | 20.9 ± 6.8 (30) |
| G653E | 2 | 85.2 ± 7.3 | 24.1 ± 7.3 (35) |
a
Dark-grown, 2.5-d-old seedlings were total hypocotyl elongation after the heat stress was measured as a percentage of the 22°C value. Mean and standard deviations were derived by measurement from three independent experiments performed with 60 or more seedlings per F3 seedling. The numbers in parentheses indicate percentage of elongation compared with that of wild-type plants.