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. 2005 Feb;17(2):572–583. doi: 10.1105/tpc.104.028522

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Copyright © 2005, American Society of Plant Biologists

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Figure 7. — Differential Localization of phH3-Ser28 and CENPC.

(A) Double labeling of CENPC (green) and phCENH3-Ser50 (red). The two signals overlap to produce a yellow color.

(B) Double labeling of CENPC (green) and phH3-Ser28 (red) at prometaphase (average kinetochore to kinetochore distance in this cell was 1.40 μm, n = 7). Note that the phH3-Ser28 lies between sister chromatids and does not overlap with the CENPC staining (arrowheads).

(C) Double labeling of CENPC (green, arrowheads) and phH3-Ser28 (red) at late metaphase (average kinetochore to kinetochore distance in this cell was 1.93 μm, n = 7). In this case, the phH3-Ser28 domain is stretched between the kinetochores as if it were under tension. Bar = 1 μm.