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. Author manuscript; available in PMC: 2018 Jun 15.
Published in final edited form as: Cancer Res. 2017 Apr 20;77(12):3306–3316. doi: 10.1158/0008-5472.CAN-17-0298

Figure 2.

Figure 2

Alb/SND1 mice develop aggressive DEN-induced HCC. WT and Alb/SND1 littermates were injected i.p. with DEN (10μg/gm) at 2 weeks of age and livers were harvested at 32 weeks post-injection when subsequent analyses were performed. A. Photographs of representative livers. Measurement of liver wt (B), and serum AST, ALT and total protein (C) (WT: n = 8; Alb/SND1: n = 10). D. Analysis of AFP and CD36 mRNA levels by Taqman Q-RT-PCR in naïve and DEN-treated livers. Normalized by GAPDH. E. Histological analysis and IHC for CD31 and PCNA in FFPE liver sections. F. Western blot analysis for the indicated proteins in the livers of DEN-treated WT and Alb/SND1 littermates at 32 weeks and spontaneous tumors in Alb/SND1 livers at one year. For graphs, data represent mean ± SEM; *: p<0.01 vs WT.