Table 3.
Primer Pairs Used for mRNA Quantification
| Primer Sequences
|
||
|---|---|---|
| Gene Names | Forward Primers | Reverse Primers |
| VGD1-specific fragment (431 bp) | 5′-CAGAGATGCTATAACCCAATCA-3′ | 5′-AGCTGCTCCTTTGTCTGAAGGA-3′ |
| At2g47030-specific fragment (323 bp) | 5′-AATGAGCACCGAGATGGGAGATT-3′ | 5′-TCATAAACCAATTTCATACACCA-3′ |
| At3g62170-specific fragment (1077 bp) | 5′-CCAGCTGGCGGTGATGATGGT-3′ | 5′-CCAGAGACGACGTCGTATCGT-3′ |