Skip to main content
. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: Cancer Res. 2010 Jan 1;70(1):57–67. doi: 10.1158/0008-5472.CAN-09-2472

Figure 6.

Figure 6

Chronic, but not acute, IGF-I depletion alters macrophage content and function in the liver. Mice were maintained on regular chow (lean) or high-calorie (obese) diets for 8 to 10 wk. RNA was extracted from the livers using Trizol and analyzed by real-time PCR. Columns, mean mRNA expression levels relative to control lean mice that were assigned a value of 1 and are based on ≥6 mice per group; bars, SEM. *, P < 0.05 relative to the respective control group that was fed the same diet. #, P < 0.05 relative to lean animals of the same genetic group. Chronic, but not acute, IGF-I depletion results in reduced expression of vascular cell adhesion molecules in the liver (C). RNA was extracted from livers of lean or obese LID, iLID, and control mice using Trizol, and expression levels of ICAM-1, VCAM-1, and P-selectin were analyzed by reverse transcription-PCR.