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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: J Immunol. 2009 Mar 15;182(6):3668–3677. doi: 10.4049/jimmunol.0803579

FIGURE 3.

FIGURE 3

ESAT-6 inhibits expression of transcription factors that positively regulate IFN-γ gene expression in human T cells. PBMC from three healthy tuberculin reactors were treated with medium alone, or with 3.3 μM ESAT-6 or CFP10 for 1 h, before adding heat-killed M. tuberculosis. After 48 h, cells were collected, total protein extracts were prepared, and the proteins were separated by SDS-PAGE (30 μg per lane). A, Western blotting was performed to evaluate the expression of CREB, ATF-2, and c-Jun. GAPDH was used as a loading control. A representative result is shown. B, Means and SD of densitometry analysis from three experiments for each transcription factor is shown.