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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: J Immunol. 2009 Mar 15;182(6):3668–3677. doi: 10.4049/jimmunol.0803579

FIGURE 7.

FIGURE 7

Effect of ESAT-6 and CFP10 on expression of early T cell activation markers. Purified CD3+ cells from PBMC of three healthy donors were incubated with medium alone, ESAT-6, or CFP10 for 1 h, then cultured with anti-CD3 and anti-CD28. After 48 h, the expression levels of CD25 (A) and CD69 (B) on CD3 + cells were analyzed by flow cytometry. Due to significant variability from donor to donor, the percentage of CD25+ or CD69+ cells obtained after stimulation with anti-CD3 and anti- CD28 was arbitrarily given a value of 1.0. Mean values and SD are shown. *, p < 0.05, compared with the cells treated with an equimolar concentration of CFP10, anti-CD3, and anti-CD28.