Table 1. Washing of RBC-lysed blood using DLD microchips resulted in high recovery of total WBCs.
| Experimenta | Fraction Analyzed | Volume (μL)b | WBC Count (×103Cells/μL)c | Total %WBC Recoveryd |
|---|---|---|---|---|
| 1 | Input | 200 | 1.5 | 97%+/−9% |
| Product | 181 | 1.6 | ||
| 2 | Input | 200 | 1.2 | 95%+/−11% |
| Product | 163 | 1.4 |
One RBC-lysed and centrifugally washed human donor blood sample was processed in duplicate experiments, performed on the same day using two separate DLD microchips.
200 μL input volume was pipetted using a hand micropipettor; standard deviation (SD), as determined by weighing, was +/−1.1 in 3 off-line measurements. Output volumes were measured using the same hand micropipettor (SD +/−1.5 in 5 off-line measurements).
Coulter Counter WBC count (SD +/−0.1 in n = 4 off-line measurements).
Calculated from Coulter Counter WBC counts and measured volumes of Input and Output (mean +/− SD; SD propagated as the square root of the sum of the squares of relative SD). The main source of error in these estimates was the number of significant digits provided by the Coulter Counter