Table 1.
Chlorophyll and Carotenoid Content of Wild-Type Seedlings during Development and Treated with Fosmydomicina
| Samples | Chlorophyll a | Chlorophyll b | Total Chlorophyll | Carotenoids |
|---|---|---|---|---|
| 3-d-old Ler | 128 ± 62.6 (100) | 48.3 ± 22.5 (100) | 175.8 ± 84.9 (100) | 60.4 ± 29.4 (100) |
| 6-d-old Ler | 368.9 ± 12.6 (288.3) | 136 ± 1.3 (281.6) | 503.5 ± 13.8 (286.4) | 146.5 ± 3.4 (242.5) |
| 18-d-old Ler | 521 ± 35.2 (407) | 206.3 ± 15.8 (427.4) | 725.4 ± 50.6 (412.6) | 198.3 ± 20.9 (328.5) |
| 8-d-old Ler MS Fos − | 280 ± 0.71 (100) | 79.2 ± 27.9 (100) | 358.1 ± 27.1 (100) | 99.8 ± 3.7 (100) |
| 8-d-old Ler MS Fos + | 127.4 ± 37 (45.5) | 25.6 ± 7.2 (32.3) | 152.5 ± 29.7 (42.6) | 41.6 ± 6.8 (41.7) |
a
Pigments were extracted from seedlings and quantified as described in Methods. Values are expressed in micrograms of pigment per gram of fresh weight. The mean of two replicates is shown ±se. In parentheses is shown the percentage of pigments in relation to the 3-d-old seedling or the wild-type without fosmidomycin (taken as 100%). The pigment level shown in this table corresponds to the 48-h treatment in liquid MS media (Fos −) or in liquid MS media plus fosmidomycin (Fos +).