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. 2017 Jun 1;15(2):136–145. doi: 10.1089/lrb.2016.0062

FIG. 1.

FIG. 1.

Effects of pharmacological blockade of NO and histamine production on imposed flow-dependent relaxation and contractile inhibition of aged MLVs. (A) Lymphatic tone index; (B) contraction amplitude; (C) contraction frequency; (D) fractional pump flow. Control, control MLVs after overnight culture; L-NAME, same control vessels after subsequent acute pharmacological blockade of NO production by the NOS nonspecific blocker, L-NAME (100 μM); a-MHD, MLVs after overnight pharmacological blockade of histamine production by the HDC-specific blocker, α-MHD at 10 μM; a-MHD+L-NAME, same α-MHD-treated vessels after subsequent acute pharmacological blockade of NO production by NOS nonspecific blocker, L-NAME in 100 μM. “*” indicates significant difference (p < 0.05) between lymphatic contractile parameters within each treatment group at no-flow and at any imposed flow conditions. “+” indicates significant difference (p < 0.05) between lymphatic contractile parameters in any treatment group and control group within any given imposed flow condition. α-MHD, α-methyl-DL-histidine dihydrochloride; HDC, histidine decarboxylase; L-NAME, Nω-nitro-L-arginine methyl ester hydrochloride; MLV, mesenteric lymphatic vessel; NOS, nitric oxide synthase.