FIG. 3.

Representative confocal images of adult (9-mo) and aged (24-mo) isolated MLVs and results of quantitative analysis of the intensity of fluorescence of the HDC signal (in red) in images taken at various settings for microscopy (40 × , 60 × , 100 × , see the Materials and Methods section for details). CD31 (PECAM-1) staining, shown in green, indicates lymphatic endothelial cell junctions and therefore delineates the location of lymphatic endothelial cells. Merged images in the third column demonstrate localization of HDC inside the lymphatic endothelial cells. A scale bar within each row of images represents 50 μm and applies to all images in that row. “#” indicates significant difference (p < 0.05) between adult and aged MLVs.