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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cancer Res. 2017 Apr 12;77(11):2927–2937. doi: 10.1158/0008-5472.CAN-16-3351

Figure 4.

Figure 4

Growth suppression of tumor cells by gene silencing. A, Inhibition of cell proliferation by knockdown of CIC-DUX4 and upregulated genes in mCDS. Relative growth of tumor cells 48 hours after siRNA treatment was calculated by comparing each cell number to cells treated with control siRNA (NC). Two oligonucleotide sequences were used for each gene and the symbols of siRNA used are indicated. B, Gene knockdown was confirmed by RT-PCR. Knockdown of CIC-DUX4 was achieved using human CIC-specific siRNAs. CIC-DUX4 of human origin was extensively downregulated (bottom left), whereas endogenous mouse Cic expression was not reduced significantly (bottom right).