Table 1.
Strains and plasmids used in this study
| Strains and Plasmids | Genotype/Properties | Reference/Resource |
|---|---|---|
| Escherichia coli | ||
| JM109 | RecA1, endA1, gyrA96, thi, supE44, relA1△ (lac-proAB)/F′ [traD36, lacI q, lacZ△M15] | Our lab’s collection |
| ED8767 | RecA, met,containing pRK2073,Spcr | Our lab’s collection |
| M15 | Kmlac ara gal mtl recA1 uvr1 [pREP4 lacI Kanr] | Our lab’s collection |
| Xanthomonos campestris pv. compestris | ||
| 8004 | Wild type; Rif r | Our lab’s collection |
| 015F07 | As 8004,but XC_3579::Tn5gusA5;Rifr, Kmr | This work |
| 045F12 | As 8004, but XC_3579::Tn5gusA5;Rifr, Kmr | This work |
| 142E11 | As 8004, but XC_3579::Tn5gusA5;Rifr, Kmr | This work |
| 0531pk | As 8004, but XC_0531::pK18mob; Rifr, Kmr | This work |
| C0531 | 0531pk harboring pLA0531; Rifr, Kmr, Tetr | This work |
| Plasmids | ||
| pK18mob | pUC18 derivative, lacZα, Kanr, mob site, suicide plasmid in X. campestris pv. campestris. | Our lab’s collection |
| pRK2073 | Helper plasmid,Tra+, Mob+, ColE1, Spcr. | Our lab’s collection |
| pET30a+ | T7 promoter, Kmr | Our lab’s collection |
| pLC0531 | pLAFR3 containing the whole XC_0531 gene;Tcr | This work |
| pLAFR3 | Broad host range cloning vector, Tcr | Our lab’s collection |