Figure 6.

Structural and functional effects on the retina following XIAP therapy. (A) XIAP therapy significantly protects photoreceptor structure following retinal detachment in comparison to the GFP-injected detached retina and XIAP-treated detached retina is not significantly different from the intact control. Hematoxylin and eosin images were imported into ImageJ, and were used to calculate the area occupied by the outer nuclear layer (photoreceptors). This was divided by the total retinal area. The analysis was conducted by a researcher who was masked to the experimental status of the animals. Averages represent four GFP animals and five XIAP animals (see Table 1 for animals included in the analysis). The intact inferior retina served as a control. The ONL area of GFP-treated detached retina is significantly reduced in comparison to XIAP-treated retina and to intact control (**p ≤ 0.01, by one way analysis of variance followed by a Bonferroni post hoc analysis). Importantly, XIAP-treated detached retina is not significantly different than intact control retina. Error bars represent SEM. (B) Scatter plot showing full-field a-wave ERG amplitudes at the endpoint of the experiment. The values between XIAP-treated and control eyes did not reach significance due to the small number of animals in the analysis (p = 0.16, using Student's t-test).