Table I.

ADK activity in leaves from Col-0 and adk1⁻/adk1⁻ mutant plants

Arabidopsis leaves from 3-week-old plants were sampled after 12 h in dark, and chloroplasts were isolated as described by Tauberger et al. (2000). ADK activity was measured in both the plastidial fraction and the supernatant with and without Triton (0.01%) following the protocol described by Kleczkowski and Randall (1986). Values are expressed in nmol min⁻¹ mg protein⁻¹ ± se and are means from three replicates. Numbers in bold type denote significant differences determined by the Student's t test (P < 0.05) from Col-0.

Triton		Col-0	Atpadk1
0.00%	Total	2.22 ± 0.05	1.84 ± 0.47
	Supernatant	0.22 ± 0.08	0.53 ± 0.06
	Plastid	2.02 ± 0.49	1.30 ± 0.68
0.01%	Total	1.53 ± 0.44	1.05 ± 0.24
	Supernatant	0.31 ± 0.08	0.48 ± 0.01
	Plastid	1.21 ± 0.09	0.56 ± 0.18