Table III.

Amino acid content in Arabidopsis roots

Wild type (Col-0) and adk⁻¹/adk⁻¹ plants were grown under various light regimes, and after 2 weeks roots were harvested, extracted, and were quantified by HPLC as described in “Materials and Methods.” Data are means of replicates ± se from six individual plants and presented as nmol g FW⁻¹. Values in bold type denote those determined to be significantly different (P < 0.05) from Col-0 by the Student's t test.

Amino Acid	Photoperiod (16/8)		Light		Darkness
	Col-0	adk−1/adk−1	Col-0	adk−1/adk−1	Col-0	adk−1/adk−1
Asp	1,280 ± 210	900 ± 100	1,000 ± 70	2,520 ± 400	1,480 ± 210	860 ± 200
Glu	2,190 ± 370	1,500 ± 280	1,500 ± 150	3,100 ± 340	2,620 ± 590	1,500 ± 280
Asn	1,200 ± 240	790 ± 160	1,000 ± 170	1,660 ± 150	3,050 ± 750	2,100 ± 450
Ser	920 ± 150	660 ± 90	610 ± 110	1,440 ± 200	1,480 ± 310	1,100 ± 300
Gln	3,110 ± 610	2,200 ± 420	2,000 ± 190	4,400 ± 680	3,130 ± 820	1,300 ± 290
Gly	110 ± 20	60 ± 5	60 ± 3	110 ± 40	60 ± 20	70 ± 20
h-Ser	20 ± 6	13 ± 4	16 ± 3	40 ± 3	80 ± 7	60 ± 10
Thr	890 ± 180	500 ± 90	750 ± 72	1,940 ± 510	1,940 ± 250	880 ± 190
His	100 ± 20	60 ± 20	70 ± 13	150 ± 20	390 ± 100	260 ± 60
β-Ala	30 ± 6	20 ± 2	30 ± 7	25 ± 1	30 ± 4	20 ± 5
Ala	540 ± 130	260 ± 90	390 ± 60	60 ± 4	750 ± 270	190 ± 30
Arg	120 ± 80	130 ± 90	70 ± 50	920 ± 160	440 ± 140	570 ± 140
GABA	1,630 ± 220	950 ± 160	1,350 ± 170	2,640 ± 360	2,240 ± 440	1,650 ± 450
Tyr	30 ± 4	30 ± 10	20 ± 1	60 ± 5	410 ± 100	260 ± 60
Val	180 ± 30	140 ± 30	120 ± 10	340 ± 20	1,180 ± 270	720 ± 160
Met	40 ± 8	30 ± 4	30 ± 2	30 ± 7	30 ± 3	13 ± 3
Trp	20 ± 2	20 ± 3	13 ± 2	16 ± 3	160 ± 50	100 ± 20
Phe	47 ± 8	30 ± 4	30 ± 2	40 ± 9	110 ± 30	80 ± 10
Ile	46 ± 7	40 ± 10	40 ± 3	100 ± 10	520 ± 120	330 ± 70
Leu	60 ± 7	70 ± 20	50 ± 4	160 ± 10	690 ± 160	380 ± 90
Lys	200 ± 50	100 ± 40	210 ± 50	110 ± 14	390 ± 90	480 ± 160