Figure 2.

Interaction studies of AtCUL3a, AtCUL3b, AtCUL4, and selected AtBPM proteins. A, AtCUL3a and AtCUL3b can both interact in Y2H assays with AtBPM1 and AtBPM3 but not with AtBPM5 and AtBPM6. AtCUL4 was used only with AtBPM1 and did not show any interaction. B, Y2H interaction studies show that AtBPM1 and AtBPM3 assemble to homodimers and heterodimers with the other tested AtBPM proteins. C, Results from the Y2H studies were confirmed for AtCUL3a, AtCUL3b, AtCUL4, and AtBPM1 by pull-down assays with bacterially expressed and purified GST or GST∷AtBPM1 proteins. First lane of each assay shows 1 μL of [³⁵S]Met-labeled protein used for pull downs. SDII, Selection medium for transformation with bait (pBTM112-D9) and prey (pACT2) plasmids supplemented with uracil and His; SDIV, selection medium for interaction studies without uracil and His supplements. Photos were taken from single spots.