Figure 1.

PAT-GPI4 is a GPI-anchored PM protein. A, Schematic organization of the PAT-GPI4 reporter protein. SP, Signal peptide; Myc, double-myc tag; black bar, 59 C-terminal amino acids of AtAGP4. B, Detection of PAT-GPI4 in MM from callus and sensitivity of PAT-GPI4 to PLC. Aqueous phase proteins of Triton X-114 partitioned MM after PLC treatment (+PLC) or mock PLC treatment (−PLC). Proteins were separated by SDS-PAGE and detected by anti-myc immunoblot. C, PM targeting. The PM-enriched fraction was prepared by dextran-PEG phase partitioning. Proteins were separated by SDS-PAGE and detected by immunoblot. A mixed organelle membrane fraction was included as control. Equal quantities of protein were loaded in each lane.