Table I.
Proteins enriched in or depleted from Arabidopsis DRMs-DIGE analysis
Proteins were separated and analyzed by 2D-DIGE. Proteins enriched in the DRM fraction labeled with red arrowheads in Figure 3B were identified by LC-MS/MS. Proteins depleted from the DRMs labeled with green arrowheads in Figure 3A were identified by comparison of DIGE gels with the 2D database of Arabidopsis organelle proteins (Prime et al., 2000).
| Spot | Protein Family | MIPS Code | Subcellular Localization |
|---|---|---|---|
| Enriched 1 | HIPL1 hedgehog-interacting-protein-like 1 | At1g74790 | PM (GPI anchor) |
| Enriched 2 | GPDL1 glycerophosphodiesterase-like protein 1 | At5g55480 | PM (GPI anchor) |
| Enriched 3 | Unknown protein | At1g29980 | PM (GPI anchor) |
| Enriched 4 | AtVHA-A V1-ATPase subunit | At1g78900 | Endomembranes/PM |
| Enriched 5 | AtVHA-B1 V1-ATPase subunit | At1g76030 | Endomembranes/PM |
| Enriched 5 | AtVHA-B2 V1-ATPase subunit | At4g38510 | Endomembranes/PM |
| Enriched 5 | AtVHA-B3 V1-ATPase subunit | At1g20260 | Endomembranes/PM |
| Enriched 6 | PAT-GPI | –a | PM (GPI anchor) |
| Enriched 7,8 | AtVHA-C V1-ATPase subunit | At1g12840 | Endomembranes/PM |
| Enriched 9 | AtFlot1 (related to flotillin) | At5g25250 | Unknown |
| Enriched 10 | Stomatin like | At4g27585 | Unknown |
| Enriched 11 | Putative quinone reductase | At4g36750 | Unknown |
| Enriched 12 | Putative hypersensitive response protein | At1g69840 | Unknown |
| Enriched 13 | Putative hypersensitive response protein | At5g62740 | Unknown |
| Enriched 14,15 | FQR1 Putative quinone reductase | At5g54500 | Unknown |
| Depleted 1 | Calreticulin | At1g56340 | ER |
| Depleted 2 | Calreticulin | At1g09210 | ER |
| Depleted 3 | Calnexin | At5g61790 | ER |
| Depleted 4 | Protein disulfide isomerase | At1g77510 | ER |
| Depleted 5 | Protein disulfide isomerase | At1g21750 | ER |
| Depleted 6 | BiP | At5g42020 | ER |
| Depleted 7 | HSP60 chaperonin | At3g23990 | Mitochondria |
| Depleted 8 | AtVSR | At2g14740 | Golgi/prevacuole |
| Depleted 9 | ATP synthase β-subunit | At5g08690 | Mitochondria |
| Depleted 10 | Peptidase | At3g02090 | Mitochondria |
| Depleted 11 | ATP synthase α-subunit | At2g07698 | Mitochondria |
| Depleted 12 | Probable ATP synthase 24-kD subunit | At2g21870 | Mitochondria |
| Depleted 13 | Cytochrome b5 | At5g53560 | ER |
| Depleted 14 | ATP synthase δ-subunit | At5g47030 | Mitochondria |
| Depleted 15 | ATP synthase δ-subunit | At3g52300 | Mitochondria |
a
PAT-GPI was specifically engineered for this study and therefore has no MIPS accession number.