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. 2017 May 23;14(1):35–42. doi: 10.3892/etm.2017.4501

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Apoptosis of cancer cell lines transfected with survivin siRNA was detected by Annexin V/PI double staining. Following siRNA knockdown of survivin in cancer cell lines (MDA-MB-231, SGC-7901, HeLa, A549, SK-OV-3, Raji and PC-3), levels of cell apoptosis were evaluated by Annexin V/PI double staining followed by flow cytofluorometry analysis. Early and late stage apoptosis (Annexin V⁺/PI⁻ and Annexin V⁺/PI⁺, respectively) in the survivin siRNA groups were increased to some extent relative to the si-NC and normal control groups. siRNA, small interfering RNA; NC, scrambled negative control siRNA; normal, untreated negative control.