Dependence of reporter response on exogenous ABA levels. LUC expression of 4-d-old pAtHB6::LUC seedlings was recorded after exposure to various ABA concentrations for 12 h. Light emission of cotyledons (A) and roots (B) in wild-type (black circles), ABA-deficient aba2-1 (white squares), and ABA-insensitive abi1-1 (white triangles) background. ABA restores the reporter response in the ABA-deficient aba2-1 mutant, whereas the response is abrogated in the abi1-1 background. Note the different saturation levels of wild-type cotyledons and roots. When related to the protein content of the samples, the saturation levels became similar for shoots and roots. Values are means of 3 independent measurements, each comprising 15 seedlings.