Figure 4.

Subcellular localization of GFP fused to the N-terminal sequences of Arabidopsis Serat proteins. Particle bombardment with chimeric GFP fusion genes was carried out with 3-week-old Arabidopsis leaves. The expression and localization of GFP was observed after 16-h incubation by confocal laser scanning microscopy. A, Plasmid pFF19-GFP used as a control for localization in cytosol and nucleus; B, GFP-carrying transit peptide of the pea ribulose-1,5-bisphophate carboxylase small subunit as a control for chloroplast translocation. C, SHMT-GFP carrying transit peptide of SHMT from Arabidopsis as a control for mitochondrion targeting; D, Serat1;1-GFP localized in cytosol as a control; E, Serat3;1-GFP localized in cytosol; F, Serat3;2-GFP also localized in cytosol.