Table II.
Effects of DCMU, PMS, DCCD, and Nig/Val on the initial rate of recovery of PSII activity
Synechocystis cells, which had been grown in light at 70 μE m−2 s−1, were exposed to light at 2,000 μE m−2 s−1 for 75 min to induce 90% inactivation of PSII. Then cells were incubated in light at various intensities in the presence or absence of various reagents. The initial rate of recovery was calculated from the time course of recovery experiments (or by fitting the recovery to a first-order reaction curve). Values are means ± sd of results from four independent experiments.
| Conditions
|
Initial Rate of Recovery min−1
|
|
|---|---|---|
| 300 μE m−2 s−1 | 600 μE m−2 s−1 | |
| No addition (control) | 0.0089 ± 0.0014 | 0.0091 ± 0.0013 |
| DCCD (10 μm) | 0.0004 ± 0.0002 | 0.0007 ± 0.0004 |
| DCMU (20 μm) | 0.0047 ± 0.0008 | 0.0046 ± 0.0009 |
| DCMU(20 μm) | ||
| DCCD (10 μm) | 0.0005 ± 0.0001 | 0.0006 ± 0.0001 |
| DCMU (20 μm) | ||
| Nigericin (2 μm) plus valinomycin (2 μm) | 0.0003 ± 0.0001 | 0.0004 ± 0.0001 |
| DCMU (20 μm) | ||
| PMS (20 μm) | 0.0073 ± 0.0009 | 0.008 ± 0.0012 |
| DCMU (20 μm) | ||
| PMS (20 μm) | ||
| DCCD (10 μm) | 0.0006 ± 0.0001 | 0.0005 ± 0.0001 |
| DCMU (20 μm) | ||
| PMS (20 μm) | ||
| Nigericin (2 μm) plus valinomycin (2 μm) | 0.0005 ± 0.0001 | 0.0005 ± 0.0001 |