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. 2005 Jan;137(1):274–286. doi: 10.1104/pp.104.050773

Figure 4.

Figure 4.

Fluorescent label for acidic pectins (monoclonal antibody JIM5) and cellulose (calcofluor white) in germinating pollen grains and corresponding DIC images. A and B, Solanum pollen grain showing intensive label for acidic pectins at all three apertures. C and D, Germinating Solanum pollen grain showing weak and evenly distributed label for cellulose. E and F, Lilium pollen grain showing very weak label for acidic pectins. The prominent label in the pollen tube serves as a control for the success of the label technique. G and H, Ungerminated and germinated Lilium pollen grains showing weak cellulose label around the grain. The ungerminated grain shows a spot of denser label (arrow) that might be the site of an emerging pollen tube. The germinated grain shows a considerable accumulation of cellulose at the base of the newly formed pollen tube. Bars = 10 μm (A–D); 30 μm (E–H).