Figure 6.
Effect of various lyticase concentrations and medium stiffness on Solanum pollen germination and pollen tube length. Germination percentage and pollen tube length were assessed after 2 h in liquid medium (♦), media solidified with 20 mg mL−1 (○), and 40 mg mL−1 (▴) agarose. Both were reduced in stiffer media, but moderate amounts of lyticase nevertheless had a significant stimulating effect. However, optimal enzyme concentrations and relative stimulation in stiff media had a tendency to differ from those observed in liquid medium, as summarized in Table II.