Table I.
Effect of cell wall-digesting enzymes lyticase, cellulase, and β-glucanase on germination percentage and pollen grain morphology in Lilium
Addition of moderate amounts of β-glucanase stimulated germination percentage and higher concentrations caused bursting, whereas neither of these effects was observed after addition of cellulase or lyticase. Relative increase (RI) is calculated as RI = (GO − GC)/GC, with GO germination at optimal enzyme concentration and GC germination at zero enzyme concentration (control).
| Enzyme
|
Stimulation of Germination
|
Inhibition of Germination
|
||
|---|---|---|---|---|
| Relative Increase Compared with Untreated Control | Optimal Enzyme Concentration | Enzyme Concentration Achieving Complete Inhibition | Pollen Grain Morphology | |
| % | mg mL−1 | mg mL−1 | ||
| Lyticase | 0 | n/a | 2 | Intact |
| Cellulase | 0 | n/a | 50 | Intact |
| β-Glucanase | 70 | 0.05 | 2 | Burst |