Table II.
Optimal lyticase concentrations that result in stimulation of Solanum pollen germination and tube length at 2 h in liquid and solidified media
Relative increase was calculated as RI = (GO − GC)/GC, with GO germination percentage or pollen tube length, respectively, at optimal enzyme concentration and GC germination percentage or pollen tube length, respectively, at zero enzyme concentration (control).
| Medium Stiffness
|
Maximal Germination at 2 h
|
Maximal Pollen Tube Length at 2 h
|
||
|---|---|---|---|---|
| Relative Increase Compared with Untreated Control | Optimal Lyticase Concentration | Relative Increase Compared with Untreated Control | Optimal Lyticase Concentration | |
| % | mg mL−1 | % | mg mL−1 | |
| Liquid | 36 | 1 | 14 | 1 |
| 20 mg mL−1 agarose | 41 | 1 | 19 | 0.5–1 |
| 40 mg mL−1 agarose | 91 | 0.5 | 100 | 0.2–0.5 |