Figure 2.

Mannose trimming is not required for the degradation of RTA. Tobacco protoplasts transfected with empty vector (pDHA) or plasmids encoding RTA*, RTA*0K, or RTA*6K were pulsed for 1 h with radiolabeled amino acids and chased for 5 h either in the absence (control) or in the presence of 5 mm DMM. Polypeptides immunoselected with anti-RTA antiserum were analyzed by SDS-PAGE and fluorography. Dot, Untrimmed RTA; arrowhead, trimmed RTA.