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Experimental protocol. Sham, IEC-6 cells were incubated in normoxic medium for 8 h; OGD/R, IEC-6 cells were incubated for 4 h in OGD medium followed by 4 h re-oxygenation; Terlipressin, 25 nM terlipressin was administrated following OGD; Wortmannin, 2 µM wortmannin was administrated following OGD; T+W, terlipressin and wortmannin were simultaneously administered following re-oxygenation. OGD/R, oxygen and glucose deprivation/re-oxygenation; T, terlipressin; W, wortmannin.
