Figure 6.

A, Immunoblot analysis of microsomal extracts for roots of 11-d-old plants of two maize genotypes differing in chilling sensitivity (Z7 (Z), tolerant and Penjalinan (P), sensitive) that have been grown at 25°C (C) or transferred for 3 d at 5°C (Ch). The blot was developed with antibodies against PIP1, PIP2, and phosphorylated PIP2 (PhPIP2) proteins (see “Materials and Methods” for details). B, Immunoblot analysis of microsomal extracts of roots; previous incubation of the nitrocelluse membrane was done with or without phosphatase (PPase). C, Immunoblot analysis of microsomal extracts for roots of 11-d-old plants of two maize genotypes differing in chilling sensitivity (Z7 (Z), tolerant and Penjalinan (P), sensitive) that have been grown at 25°C (C) or after 1 h of exposure of the roots to 100 μm of H₂O₂ (H). The blot was developed with antibodies against PIP1, PIP2, and phosphorylated PIP2 (PhPIP2) proteins (see “Materials and Methods” for details).