Table II.
Sequences of the PCR primers used to amplify the specific PIP fragments used as probes in the northern blots
Name | Accession No. | Forward Primer | Reverse Primer | Fragment Position |
---|---|---|---|---|
ZmPIP1;1 | X82633 | PIP1;1-1 | PIP1;1-2 | 922–1,159 |
5′-TAAAGGAGCCGATGCTGCTG-3′ | 5′-GGATGAACTCTTAAAGCTTGAC-3′ | |||
ZmPIP1;2 | AF131201 | PIP1;2-1 | PIP1;2-2 | 972–1,273 |
5′-GCGTCTTCCTGTGATGTCTTCT-3′ | 5′-AAATCAAGAAAACCCTGAATCG-3′ | |||
ZmPIP1;3 | AF326487 | PIP1;3-1 | PIP1;3-2 | 1,023–1,283 |
5′-GTCTTCCTGTGGGGAGTGTCTT-3′ | 5′-GCTGATAGATAAACCCACGTTTCC-3′ | |||
ZmPIP1;4 | AF326488 | PIP1;4-1 | PIP1;4-2 | 846–1,140 |
5′-CCATCATCTACAACCGGGATCA-3′ | 5′-ACACACAGCTCGGTACAGGAGT-3′ | |||
ZmPIP1;5 | AF326489 | PIP1;5-1 | PIP1;5-2 | 957–1,243 |
5′-ATTACCAACAGCAACCATGCAG-3′ | 5′-CTTCACCGTACCAAAACCCAAG-3′ | |||
ZmPIP1;6 | AF326490 | PIP1;6-1 | PIP1;6-1 | 1,103–1,280 |
5′-CTTGCCGTCATACACGGAGTTC-3′ | 5′-ATTGGGACACGTACAGCCACAT-3′ | |||
ZmPIP2;1 | AF326491 | PIP2;1-1 | PIP2;1-2 | 872–1141 |
5′-CGGCCTTCTACCACCAGTACAT-3′ | 5′-CATGATTACATTGCAGGGGAAC-3′ | |||
ZmPIP2;2 | AF326492 | PIP2;2-1 | PIP2;2-2 | 851–1,234 |
5′-CGTCTACAACAAGGACAAGCCA-3′ | 5′-CACACATGGTGATTGATGATTAC-3′ | |||
ZmPIP2;3 | AF326493 | PIP2;3-1 | PIP2;3-2 | 926–1,101 |
5′-CTACCGGAGCAACGCCTAATAA-3′ | 5′-CCCGTCAAGGAAAGAAAGAAAA-3′ | |||
ZmPIP2;4 | AF326494 | PIP2;4-3 | PIP2;4-4 | 936–1,168 |
5′-CTACCGGAGCAACGCCTAA-3′ | 5′-ATCGGATAAAAACTCACGCAAT-3′ | |||
ZmPIP2;5 | AF130975 | PIP2;5-1 | PIP2;5-1 | 759–1,076 |
5′-GCGCTGCTGTCATCTACAACAA-3′ | 5′-GCAAGCAAAATGCAGTGGAAAT-3′ | |||
ZmPIP2;6 | AF326495 | PIP2;6-1 | PIP2;6-2 | 887–1,182 |
5′-CGCTATACCACCAGATCGTCCT-3′ | 5′-AGTCACACTTGCTTTCCACACC-3′ | |||
ZmPIP2;7 | AF326496 | PIP2;7-3 | PIP2;7-4 | 1–141 |
5′-GCACGAGCACACACATAGTAGC-3′ | 5′-CGGAGGAGGGTCATGGTAGTC-3′ |