Figure 10.

Transcript accumulation of genes of octadecanoid biosynthesis in stems of Sitka spruce after feeding by white pine weevil or treatment with MeJA. RNA for northern hybridizations was isolated from outer stem samples harvested over a time course of 32 d after treatment of sapling trees with 0.1% Tween 20 (Tween), treatment with 0.01% MeJA in 0.1% Tween (MeJA), or after feeding by white pine weevils (Weevil). EST cDNA clones used as probes are described in Supplemental Table VI. PLA, phospholipaseA; LOX, lipoxygenase; AOS, allene oxide synthase; AOC, allene oxide cyclase; OPDAR, 12-oxo-phytodienoic acid reductase. Ethidium bromide-stained major ribosomal RNA bands serve for evaluation of equal gel loading.