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. 2017 Jun 9;13(6):932. doi: 10.15252/msb.20167490

Figure EV5. ANKRD55 localization and movement along axonemes is consistent with being a component of the IFT‐B particle.

Figure EV5

  1. Two‐color kymograph generated by co‐expression of ANKRD55‐GFP (green) and mCherry‐CLUAP1 (magenta) reveals that ANKRD55 travels along axonemes in association with other IFT proteins. Scale bar: 10 μm. Kymograph is representative out of 22 multi‐ciliated cells.
  2. RT–PCR demonstrates the efficiency of ANKRD55 MO to disrupt splicing of ANKRD55 mRNA in Xenopus embryos. GAPDH is used as a control.
  3. Morpholino knockdown of JBTS17, known to specifically affect IFT‐B localization, results in the accumulation of ANKRD55‐GFP in axonemes (green: ANKRD55‐GFP, magenta: membrane RFP). Scale bar: 10 μm. Each image is representative of 18 cells from six different embryos.